Coding
PBS-vioE

Part:BBa_K323131:Design

Designed by: Tina Lebar, Tjaša Stošicki   Group: iGEM10_Slovenia   (2010-10-20)

PBSII_vioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The PBSII zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioE gene was obtained by PCR from an existing BioBrick of violacein operon (Part:BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.

Source

The vioE sequence was obtained by PCR from an existing part, which was designed by the Cambridge 2009 iGEM team (Part:BBa_K274002). The PBSII zinc finger is a synthetic gene, which our team designed and ordered from GeneArt.

References